Part:BBa_K769020:Design

lux operon(K769011) + lumP(K769019)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal NheI site found at 1680
  Illegal NheI site found at 5955
  Illegal NheI site found at 8546
  Illegal NheI site found at 8569
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6417
  Illegal BglII site found at 8936
  Illegal BamHI site found at 1144
  Illegal XhoI site found at 3767
  Illegal XhoI site found at 4275
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 1965
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1859
  Illegal BsaI.rc site found at 2541
  Illegal SapI site found at 961
  

Design Notes

First, we produced the constitutive promoter + lumP + double terminator construct(BBa_K769019).



Then, we combined the construct to the construct of cloned Lux operon with Pbad promoter.

Source

Photobacterium phosphoreum